| 摘要 |
A pure or recombinant protein preparation (I) of human telomerase reverse transcriptase (hTRT), a ribonucleoprotein, or its variants are new. Also claimed are: (1) isolated, synthetic, pure or recombinant polynucleotides that encode (I); (2) use of polynucleotide (II) (10 nucleotide to 10 kb) that has contiguous sequences complementary to naturally occuring hTRT gene or mRNA, in screening of the gene or mRNA or to prepare a recombinant host cell; (3) a cell containing (II); and (4) an antibody against hTRT. The following methods are also claimed: (A) determining whether a test compound is a modulator of hTRT, by detecting the change in hTRT recombinant protein or polynucleotide, on administration of the compound; (B) preparation of recombinant telomerase by contacting (I) with a telomerase RNA component; (C) detection of the hTRT RNA or protein in a sample by binding a relevant probe to the sample and detecting the complex formed or in the case of RNA detection, amplifying the product and correlating the presence of complex or amplification product with presence of hTRT in the sample; and (D) increasing the proliferation of a vertebrate cell by increasing hTRT expression; and (E) the use of an agent that causes an increase in cell vertebrate cell proliferation to create a medicament that inhibits ageing. #CMT#USE : #/CMT# (I) and (II) can be used in the manufacture of medicaments for inhibiting the effect of ageing or cancer. Inhibitors of telomerase activity can be used to treat conditions that are associated with high telomerase activity (all claimed). (I) can also be used in the new methods. |
