发明名称 |
Method for assaying DNA fragments in mixture |
摘要 |
The inventive method for assaying DNA fragments in mixture comprises step 1 of ligating different oligomers hybridizable to primers of the same melting temperature and the same length to individual groups of DNA fragments in a set of DNA fragments; step 2 of mixing together the groups of DNA fragments ligated with the oligomers; step 3 of simultaneous PCR of the groups of DNA fragments ligated with the oligomers in one receptacle by using the primers being complementary to the oligomers and corresponding to the individual groups; and step 4 of detecting PCR amplified DNA fragments; characterized in that the method enables the comparison of plural samples under no influence of PCR reproducibility.
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申请公布号 |
US2002098490(A1) |
申请公布日期 |
2002.07.25 |
申请号 |
US20010842791 |
申请日期 |
2001.04.27 |
申请人 |
HITACHI, LTD. |
发明人 |
UEMATSU CHIHIRO;KAMBARA HIDEKI;OKANO KAZUNORI |
分类号 |
C12N15/09;C12Q1/68;G01N33/53;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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