发明名称 |
Methods to overexpress a foreign gene in a cell or in an animal in vitro and in vivo |
摘要 |
The present invention provides a nucleic acid molecule comprising: (a) a region of DNA which is homologous to a region of an endogenous gene present in a genome of a cell of interest; (b) a first nucleic acid encoding an encephalomyocarditis internal ribosome entry site (EMCV IRES); (c) a second nucleic acid encoding a selectable marker which can be excised from the nucleic acid molecule if the nucleic acid molecule has been integrated into the genome of the cell of interest; and (d) a third nucleic acid encoding a gene of interest. The cell may be an animal cell, a yeast cell or a plant cell. The invention also provides for transgenic non-human animals which are created using the above described construct. The invention also provides methods for making such transgenic animals.
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申请公布号 |
US2002099194(A1) |
申请公布日期 |
2002.07.25 |
申请号 |
US20010905311 |
申请日期 |
2001.07.13 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY |
发明人 |
EFSTRATIADIS ARGIRIS;LUDWIG THOMAS;KLJUIC ANA;POLITI KATERINA |
分类号 |
C12N15/85;(IPC1-7):A01K67/027;C07H21/04;C12N15/00 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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