METHODS AND REAGENTS FOR AMPLIFICATION AND MANIPULATION OF VECTOR AND TARGET NUCLEIC ACID SEQUENCES

摘要

The invention provides methods and compositions for the amplification of vector sequences, particularly for amplification of vectors applied to the elucidation of mammalian gene function. The present invention relates to methods and compositions for recovery / amplification of DNA sequences from mammalian complementation screening products, products of the functional inactivation of specific essential or non-essential mammalian genes, and products from the identification of mammalian genes which are modulated in response to specific stimuli. The methods and compositions of the present invention are applicable (but are not limited) to recovery of replication-deficient retroviral vectors, libraries comprising such vectors, retroviral particles produced by such vectors in conjunction with packaging cell lines, integrated provirus sequences derived from the retroviral particles of the invention and circularized provirus sequences which have been excised from the integrated provirus sequences of the invention. The compositions of the present invention further include novel retroviral packaging cell lines.