Process for preparing expression vector for producing therapeutic proteins in transgenic animals

摘要

The solution refers to the way of vector construction, based on the utilization of modified translational Kozak consensus to connect promoter regulatory sequence with a coding structural gene fragment. The recombinant plasmid vector prWhEBS-BNX was constructed. For connection of the gene genome sequence for hEPO with the promoter of gene for rWAP the original motif of translation Kozak consensus is modified by installation of NotI restriction site. In this way prepared expression vector prWhEBS-BNX is transferred to genome of eukaryotic cells and genome of vertebrates for production of a polypeptide product presenting the entire primary structural conformation of human erythropoietin.