| 摘要 |
PURPOSE: Provided is HIV like particle which is composed of HIV-1 Gag, Env, Pol and/or Pro proteins expressed by an expression vector containing HIV-1 env, gag, pro, pol genes. The HIV like particle can be used as an antigen in a diagnosis kit of HIV infection and for HIV vaccine composition. CONSTITUTION: Alphavirus based expression vectors contain HIV-1 env, gag, pro and pol genes and they are as follows: pSFV/env-gag, pSFV/gag-env, pSFV/env-gag-pro, pSFV/env-gag-gagpro, pSFV/env-gag-gagΔpro, and pSFV/gagpol-env. The expression vectors further contains CTE(constitutive transport element) gene and they are as follows: pSFV/env-gag-gagpro-CTE, pSFV/env-gag-gagΔpro-CTE, pSFV/gagpol-env-MCTE, and pSF/gag-env-MCTE. HIV-like particle is manufactured by introducing one of the above vectors or its RNA transcript into an animal cell then expressing it.
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