发明名称 |
THERMOPHILIC PROLYLENDOPEPTIDASE |
摘要 |
PROBLEM TO BE SOLVED: To obtain a thermophilic prolylendopeptidase useful for structural analysis of proteins. SOLUTION: This isolated DNA contains a nucleotide sequence encoding a polypeptide having thermophilic prolylendopeptidase activity. This recombinant vector contains the DNA. This expression vector contains the DNA. This transformant is transformed by the expression vector. This method for producing the polypeptide by using the transformant is provided. A formed prolylendopeptidase builds up an essential protein structure by heat treatment at a high temperature for a fixed time and can actualize high activity. The thermophilic prolylendopeptidase is obtained from Pyrococcus horikoshii. |
申请公布号 |
JP2002199888(A) |
申请公布日期 |
2002.07.16 |
申请号 |
JP20010000840 |
申请日期 |
2001.01.05 |
申请人 |
AGENCY OF IND SCIENCE & TECHNOL;RAKUTO KASEI INDUSTRIAL CO LTD;NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL & TECHNOLOGY |
发明人 |
SHIMOMURA AKIRA;ANDO SUSUMU;KOSUGI YOSHIJI;ISHIKAWA KAZUHIKO;ISHIDA HIROYASU |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/52;C12R1/01 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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