| 摘要 |
PURPOSE: Provided is a transformation vector for chloroplast of the moss Physcomitrella patens, thereby mass-producing foreign proteins in a plant cell. CONSTITUTION: A recombinant vector, pMAI-X, is contains trn I, and trn A genes derived from chloroplast of the moss Physcomitrella patens, and prrn, aadA, and psbA3 genes derived from pCT-V2 vector for the transformation in tobacco. It is manufactured by the steps of: cleaving the nucleotide sequence of SEQ ID NO:1, which is sequenced by amplifying trn I and trn A gene form Physcomitrella patens genome, with dIII; inserting the cleaved nucleotide sequence into dIII site of pBluescript SK(+) to manufacture pCS629; separating prrn, aadA, and psbA3 from pCT-V2 then inserting them into Nsi site of pCS629 to manufacture pMAI; cleaving a nucleotide sequence containing XbaI cleavage site, in pMAI vector, with restriction enzymes, EcoRI and NotI; and treating cleaved site with Klenow fragment to fill-in then self-ligating it.
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