METHOD OF REGULATING TRANSCRIPTION IN A CELL BY ALTERING REMODELING OF CROMATIN

摘要

The present invention provides a method of altering remodeling of chromatin in a cell comprising administering to the cell a compound that modulates an interaction of a subunit of a chromatin remodeling complex and a domain within a protein. The compound modulates the interaction by inhibiting or enhancing the subunit of the chromatin remodeling complex and the domain within the protein. The protein may be a transcription factor. The domain may be a nucleic acid binding domain or an activation domain. The nucleic acid binding domain, such as peptide or an artificial zinc-finger domain. The first peptide may be linked to a second protein to form a fusion protein. The nucleic acid may be DNA or RNA. The chromatin remodeling complex may be SWI/SNF, RSC, NURF, CHRAC, ACF, NURD and RSF. The remodeling complex may be tissuespecific. In particular the chromatin remodeling complex may be one of the following SWI/SNF subunits: BRG1, BRM, BAF 155, BAF 170, Inil, BAF 60, BAF 47, or BAF 57. The subunit may be associated with at least one other subunit. The SWI/SNF complex may be E-RC1.