METHOD FOR IDENTIFYING NUCLEIC ACID BASE PHOSPHORAMIDITES BY FLUORESCENCE SPECTROSCOPY

摘要

Methods for detecting and identifying individual nucleic acid base phosphoramidites in a sample solution by matching fluorescent excitation and/or emission spectra to similar spectra of known phosphoramidites are disclosed. Methods of determining the accuracy of nucleic acid synthesis by analyzing fluorescent spectra of samples (influent, effluent, reaction mixtures) associated with individual coupling steps that use nucleic acid base phosphoramidites andidentifying the phosphoramidite contained in each sample are disclosed.