Embryogenesis process for initiation

摘要

The present invention is a method of producing mature somatic conifer embryos at a higher yield. Explants of immature embryos are placed on or in an initiation medium or on a nurse culture which is on or in the initiation medium. Over a period of time, initiation takes place and the initiated embryos are ultimately matured on an appropriate maturation medium. The initiation medium and the maturation medium may be the same or different but the initiation medium must contain at least ABA and at least one amino acid or at least one amino acid and no ABA. The maturation medium must contain ABA and at least one amino acid. Advantages of the present invention include ensuring greater efficiency in both initiation and maturation of any initiated embryogenic tissue.