PROCESS FOR ENZYMATICALLY PREPARING 7-AMINOCEPHALOSPORANIC ACID

摘要

PURPOSE: A process for enzymatically preparing 7-aminocephalosporanic acid is provided, thereby preparing high purity 7-aminocephalosporanic acid in higher yield. CONSTITUTION: A process for enzymatically preparing 7-aminocephalosporanic acid comprises the steps of: reacting cephalosporin C with immobilized D-amino acid oxidase to prepare 7-aminocephalosporanic acid (GL-7-ACA) and ketoadipyl 7-aminocephalosporanic acid (Keto-7-ACA); and reacting GL-7-ACA with GL-7-ACA acylase, wherein the initial pH is maintained during the reaction of cephalosporin C and D-amino acid oxidase and hydrogen peroxide is added into the reaction solution of cephalosporin C and D-amino acid oxidase when pH of the reaction solution is increased to 0.1 or more to convert remaining Keto-7-ACA to GL-7-ACA; pH of the reaction solution is maintained 7.1 to 8.0; and the concentration of hydrogen peroxide is 5 to 10%.