Fremgangsmåde til amplificering af specifikke nucleinsyresekvenser

摘要

The present invention provides a method of detecting a genetic polymorphism in an individual. In one form, the method comprises the following steps: (1) obtaining a sample containing a nucleic acid from the individual; (2) amplifying the nucleic acid sample from step (1) by a process involving thermocycling and primers, the amplification occurring in the presence of a thermostable restriction endonuclease which retains activity during thermocycling, the primers being selected such that they introduce into either the nucleic acid amplified from nucleic acid not including the polymorphism or from nucleic acid including the polymorphism, a sequence recognized by the thermostable restriction endonuclease; and (3) analyzing the product of step (2) to determine the presence of the polymorphism.