| 摘要 |
A method for screening a substrate of an ATP-binding cassette (ABC) protein characterized by comprising mixing and incubating a cell membrane fraction with the expression of the ABC protein, a labeled ATP, vanadic acid and a test sample and adding the resultant mixture to a support having an antibody against the ABC protein immobilized thereon, or mixing and incubating the labeled reagent, the test sample, etc. on the support, and then assaying the immobilized label or the unimmobilized label; and a kit for screening a substrate or an inhibitor of the ABC protein which contains a cell membrane fraction with the expression of the ABC protein, a labeled ATP or its derivative, and a support having an antibody against the ABC protein immobilized thereon, or an antibody against the ABC protein and a support.
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