PROCESS FOR PRODUCING RECOMBINANT PROTEIN AND FUSED PROTEIN

摘要

It is intended to provide an expression system of a recombinant protein and a cell-free translation system with the use of a host wherein a target protein is surely incorporated into the stereo structure of chaperonin as a fused protein with a chaperonin subunit (namely, a molecular chaperonin of about 6 0 kDa, heat shock protein of 60 kDa or thermosome) to thereby inhibit the expression of toxicity of the target protein in the host, the formation of a n enclosed matter, and digestion with a protease, thereby universally expressi ng any protein as a soluble protein. Namely, a process for producing a protein characterized by transcribing and translating a gene containing a gene encoding a chaperonin subunit and a gene encoding a target protein to thereb y synthesize a fused protein in which the target protein is linked to the chaperonin subunit via a peptide bond.