| 摘要 |
The present invention relates to a method of screening a potential translational regulatory element of mRNA, which promotes or suppresses the translation efficiency of mRNA in a given translation system, by applying the in vitro evolution principles. Specifically, the present invention relates to a method of screening a potential translational regulatory element of mRNA, comprising the steps of synthesizing mRNAs with random oligonucleotide sequences, which are candidates of translational regulatory elements, introduced into the untranslated regions (UTRs), and selecting mRNAs with altered translation efficiency by virtue of the inserted motifs; to translational regulatory elements screened by this method; and to a method of isolating mRNA with altered translation efficiency in comparison with the native mRNA by using such a screening method.
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