Absorbance multiplex technology

摘要

A method of simultaneously detecting and quantitating multiple target molecules in a single reaction well of a reaction surface. Antibodies to different target molecules are bound to a reaction surface. A sample solution believed to contain the target molecules in added to the reaction surface and incubated. During incubation, the target molecules from complexes with the bound antibodies. After a washing step, an enzyme-bound antibody mixture is added to the reaction surface. The enzyme-bound antibodies have an affinity for the target molecule/bound antibody complexes. The sequential addition of specific enzyme substrates permits the detection of multiple target molecules without poisoning any of the enzymes. A kit for the simultaneous detection of multiple target molecules is also disclosed.