发明名称 |
BACILLUS AMYLOLIQUEFACIENS CHO104 WITH ANTAGONISTIC PROPERTY AND ANTIMICROBIAL COMPOUND PRODUCED THEREBY |
摘要 |
PURPOSE: Bacillus amyloliquefaciens CHO104 with antagonistic property and an antimicrobial compound produced thereby are provided. The novel antimicrobial compound can be useful in medicines, agricultural chemicals, foods, etc. CONSTITUTION: The microorganism Bacillus amyloliquefaciens CHO104(KCTC 18060P) isolated from the soil produces an antimicrobial compound. A microbial preparation containing Bacillus amyloliquefaciens CHO104 is prepared by culturing Bacillus amyloliquefaciens CHO104 in SDBP(soy dextrose beef ex. proteose peptone) medium at 30 to 35 deg. C. The novel antimicrobial compound, Amyloliquetone, is produced by the steps of: separating the supernatant of the cultured medium of Bacillus amyloliquefaciens CHO104; collecting an ethylacetate-soluble acidic·neutral fraction from the supernatant by adding 1N HCl; collecting an ethylacetate-soluble neutral fraction by adding 2 to 5% NaHCO3; subjecting the ethylacetate-soluble neutral fraction to an adsorption chromatography on silica gel to obtain an active fraction; and subjecting the active fraction to octadecylsilane column chromatography and HPLC, sequentially.
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申请公布号 |
KR20020051803(A) |
申请公布日期 |
2002.06.29 |
申请号 |
KR20000081428 |
申请日期 |
2000.12.23 |
申请人 |
CHO, JUNG IL;PARK, KEUN HYUNG |
发明人 |
CHO, JEONG YONG;CHO, JUNG IL;LEE, SEUNG JE;PARK, KEUN HYUNG |
分类号 |
C12N1/20;(IPC1-7):C12N1/20 |
主分类号 |
C12N1/20 |
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