| 摘要 |
FIELD: veterinary science. ^ SUBSTANCE: method includes intracutaneous injection of PPD-tuberculin in dose of 0.2 ml to animals into right part of neck with simultaneous injection of atypical mycobacteria complex (AMC) into left part of neck, accounting and evaluation of reaction to tuberculin and AMC in 72 hours by measurement of skin fold of injection spot, division of animals into groups that "do not react to tuberculin and AMC", "react to AMC (-)", "react to tuberculin (+)", and "react to tuberculin and AMC (=)" and definition of statistical validity of differences in reactions to tuberculin and AMC, on the basis of which conclusion is made on infection of animals with mycobacteria of tuberculosis or atypical mycobacteria. Additionally each animal of diagnosed group independently from its diagnostic index "do not react to tuberculin and AMC", "react to AMC (-)", "react to tuberculin (+)", and "react to tuberculin and AMC (=)" is exposed to venous blood draw on an empty stomach with immediate injection of solution of hydrocortisone-acetate in dose of 5 ml intramuscularly with further draw of venous blood in 3 and 6 hours for the possibility to detect indices of activity of blood serum ferment - lactate dehydrogenase (LDG) in dynamics. Afterwards indices of LDG activity in dynamics are detected, average indices of LDG fermentative activity are compared in dynamics, those that are defined in group of animals who "do not react to tuberculin and AMC", to indices of fermentative activity of each animal in all other groups. If LDG activity increases in 3 hours by less than 8%, and in 6 hours by less than 17%, compared to average indices of fermentative activity of LDG, animals that "do not react to tuberculin and AMC", "react to AMC (-)", "react to tuberculin (+)", and "react to tuberculin and AMC (=)" are included in group of truly tuberculosis diseased animals of diagnosed group. ^ EFFECT: method makes it possible to improve accuracy of tuberculosis diagnostics. ^ 2 tbl, 1 ex, 1 dwg |
