发明名称 |
MONOCLONAL ANTIBODY PRODUCTION BY EBV TRANSFORMATION OF B CELLS |
摘要 |
A method for producing a clone of an immortalised human B memory lymphocyte, comprising the step of transforming human B memory lymphocytes using Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalised human B memory lymphocyte capable of producing a human monoclonal antibody with a desired antigen specificity, comprising the steps of: (i) selecting and isolating a human memory B lymphocyte subpopulation; (ii) transforming the subpopulation with Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator; (iii) screening the culture supernatant for antigen specificity; and (iv) isolating an immortalised human B memory lymphocyte clone capable of producing a human monoclonal antibody having the desired antigen specificity. |
申请公布号 |
US2016160258(A1) |
申请公布日期 |
2016.06.09 |
申请号 |
US201615018688 |
申请日期 |
2016.02.08 |
申请人 |
Institute For Research in Biomedicine |
发明人 |
Lanzavecchia Antonio |
分类号 |
C12P21/00;C07K16/10 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
1. A method for producing an anti-RSV monoclonal antibody comprising:
(i) contacting a memory B lymphocyte with EBV and an agonist of a Pattern Recognition Receptor that is expressed on memory B cells; (ii) culturing said memory B lymphocyte to produce a monoclonal antibody, wherein said monoclonal antibody is directed against respiratory syncytial virus (RSV). |
地址 |
Bellinzona CH |