发明名称 |
Nucleic acid integration in eukaryotes |
摘要 |
The invention relate to the field of molecular biology and cell biology. It particularly relates to methods to direct integration of a nucleic acid of interest towards homologous recombination and uses thereof. The present invention discloses factors involved in integration of a nucleic acid by illegitimate recombination which provides a method to direct integration of a nucleic acid of interest to a pre-determined site, whereby said nucleic acid has homology at or around the said pre-determined site, in an eukaryote with a preference for non-homologous recombination comprising steering an integration pathway towards homologous recombination. Furthermore, the invention provides a method to direct integration of a nucleic acid of interest to a sub-telomeric and/or telomeric region in an eukaryote with a preference for non-homologous recombination. |
申请公布号 |
EP1217074(A1) |
申请公布日期 |
2002.06.26 |
申请号 |
EP20000204693 |
申请日期 |
2000.12.22 |
申请人 |
UNIVERSITEIT LEIDEN;STICHTING BINAIR VECTOR SYSTEEM |
发明人 |
HOOYKAAS, PAUL JAN JACOB;VAN ATTIKUM, HAICO;BUNDOCK, PAUL |
分类号 |
C12N15/81;C12N15/82;C12N15/90 |
主分类号 |
C12N15/81 |
代理机构 |
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地址 |
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