发明名称 |
Polypeptides for the detection of microtubule depolymerization inhibitors |
摘要 |
This invention provides methods for the screening and identification of agents having potent effects on the progression of the cell cycle. In one embodiment, the methods involve contacting a polymerized microtubule with a microtubule severing protein or a microtubule depolymerizing protein in the presence of an ATP or a GTP and a test agent; and detecting the formation of tubulin monomers, dimers or oligomers. The p60 subunit of katanin provides a particularly preferred microtubule severing protein possessing both ATPase and microtubule severing activities.
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申请公布号 |
US6410687(B1) |
申请公布日期 |
2002.06.25 |
申请号 |
US19990291170 |
申请日期 |
1999.04.13 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
VALE RONALD D.;HARTMAN JAMES J. |
分类号 |
C12N1/21;C12N9/14;C12Q1/42;G01N21/64;G01N33/48;G01N33/483;G01N33/50;G01N33/52;G01N33/53;G01N33/68;(IPC1-7):C07K1/00;C07K14/00;C07K17/00;C07K16/00;A61K35/14 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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