Method and System for Determination of Molecular Interaction Parameters

摘要

A method of determining kinetic parameters for a reversible molecular interaction between a ligand immobilized to a solid support surface and a binding partner to the ligand in solution, comprises sequentially, without intermediate regeneration or renewal of the immobilized ligand, flowing a plurality of fluid volumes containing different known concentrations of the binding partner over the solid support surface, monitoring the momentary amount of binding partner bound to the solid support surface related to time and solution concentration of binding partner and collecting the binding data, and determining the kinetic parameters by globally fitting a predetermined kinetic model for the interaction between the binding partner and the immobilized ligand to the collected binding data, which model allows for mass transport limitation at the solid support surface. An analytical system for carrying out the method, a computer program, a computer program product and a computer system for performing the method are also disclosed.

主权项

1. An analytical system for monitoring interactions between molecules comprising:
an interaction detector for detecting a reversible molecular interaction between i) a ligand immobilized to a solid support surface and ii) a binding partner to the ligand in a predetermined concentration in a fluid at the support surface; a microfluidic system arranged to provide a flow of the fluid to the interaction detector support surface, the microfluidic system further comprising: a sample solution inlet for introducing said binding partner into the system; a buffer solution inlet; a tubing segment fluidically connecting the sample solution inlet and the buffer solution inlet to the interaction detector, and a controller arranged to control the supply of sample solution and buffer solution into the tubing segment to form a mixture of buffer and sample solution and thereby to form said fluid having the predetermined concentration of the binding partner at the interaction detector support surface.