| 摘要 |
A method and apparatus for analysis of multiplexed samples, for example of DNA, for detection of components of interest, for example single nucleotide polymorphisms (SNPs), involves tagging a number of the samples with respective fluorescing markers, for example up to four markets, mixing the tagged samples (12) and passing the mixture through a separation stage, such as a denaturing high performance liquid chromatography (DHPLC) column (14). Effluent (22) from the separation stage (14) is passed through a flow cell (20) of a fluorometer (18) wherein it is cylically subjected to pairs of excitation and emission wavelengths (30 and 32) to sequentially stimulate each marker - DNA sample combination and detect the resultant fluorescence. Each fluorescence measurement and time thereof are recorded via a computer (36) from which the fluorescence for each wavelength pair as a function of time may be plotted to provide separate chromatograms for each respective marker - DNA sample combination from which a SNP in each DNA sample is determinable. The invention provides a considerable increase in the speed at which specimens can be analysed. For commercially acceptable performance of the invention, a pulsed high intensity light source (24), for example a Xenon flash lamp, is required for the excitation light (26) together with high speed exchange of the pairs of optical filters (30 and 32) for the pairs of excitation and emission wavelengths. |
