SUPPORT POUR DETERMINER UN ANALYTE TEL QU'UNE CIBLE ADN OU ARN, COMPORTANT UN FILTRE SPECTRAL SELECTIF

摘要

The invention concerns an analysis support for determining an analyte such as a target DNA or RNA, by using a specific binding reaction (hybridization) of said analyte with a ligand specific of said analyte, and determining the binding reaction using at least two fluorescent markers present on the analyte. Said determination is carried out by applying an excitation field and by detecting the fluorescent emission of the different fluorescent markers. The invention is characterised in that the support comprises a substrate coated with one or several material layers forming an assembly capable of attenuating or increasing the excitation field for at least one of the fluorescent markers relative to the others. The ligands are fixed on the last layer of the assembly. A SiO2 layer on a silicon substrate can be used with the Cy3 and Cy5 fluorescent markers.