| 摘要 |
<p>An immunoassay characterised in that the method excludes washing steps and comprises initially diluting a sample containing an analyte to be tested, with a first reagent having a haemolysing property upon blood cells in the test sample thus preventing aggregation thereof, introducing a solid phase to the test sample mixture on which there is coupled a first immunoreactant, introducing a second immunoreactant, and further introducing a substrate/visualisation complex capable of binding the labelled immunoreactant to form a conjugate; wherein said substrate/visualisation complex is diluted by a second reagent, the second reagent comprising a mixture of phosphate buffer, protein and detergent, said reagent adapted to improve interaction between the solid phase and the substrate whilst also optimising visualisation of the analyte of interest.</p> |
