Ligand grafted substrates

摘要

Ligand-functionalized substrates are describe that are useful in selectively binding and removing biological materials from biological samples, and methods for preparing the same.

主权项

1. A method for preparing a ligand-functionalized substrate comprising:
a) providing a base substrate; b) providing a coating solution comprising a copolymer of one or more monomer units having a photoinitiator group, one or more monomer units having a crosslinkable functional group, one or more hydrophilic monomer units, and optionally one or more “other” monomer units; c) coating the base substrate with the coating solution; d) crosslinking the coating to produce a crosslinked copolymer; e) imbibing the substrate bearing the crosslinked copolymer with a solution or suspension comprising (a) one or more ligand functional monomers, (b) optionally one or more hydrophilic monomers; and optionally c) one or more “other” monomers; f) exposing the imbibed base substrate to UV radiation so as to generate free radicals from the photoinitiator groups of the copolymer, and g) graft-polymerizing the monomers of step e) onto the surface of the base substrate.