KIT FOR DETECTING OR ASSAYING SUBJECT SUBSTANCE AND DETECTION OR ASSAY METHOD

摘要

A kit and a production method therefor are provided, which kit can be produced in a high yield, can easily label ligands and can control the sensitivity of reagents. A kit for detecting or measuring an analyte having bivalent or higher binding capability includes the following receptor I, receptor II, and solid phase conjugate. Receptor I: L1-B1-R1-M including a compound R1-M bound to a compound L1-B1, in which the compound R1-M includes a substance R1 being capable of binding to a substance B1 bound to a marker M, the compound L1-B1 includes a ligand L1 and the substance B1. Receptor II: L2-B2-R2-B3 including a compound L2-B2 previously bound to a compound R2-B3, in which the compound L2-B2 includes a ligand L2 capable of binding to the analyte A and a substance B2 being introduced into the ligand L2 and having different binding capability from the analyte A, the compound R2-B3 includes a substance R2 capable of binding to the substance B2 and being bound to a bond element B3 having different binding. Solid phase conjugate: R3-solid phase including an anti-bond element R3 bound to a solid phase, in which the anti-bond element R3 is capable of binding to the bond element B3. <IMAGE>