HUMAN INTERFERON-.BETA.2A AND INTERFERON-.BETA.2B; VECTORS CONTAINING GENES CODING FOR SAID INTERFERONS: CELL LINES PRODUCING SAME AND USE OF SAID INTERFERONS AS PHARMACEUTICALS

摘要

Human interferon-beta2A and interferon-beta2B are produced in purified form by recombinant DNA techniques. Two separate human genes have been identified which code for the production of IFN-.beta.2A and IFN- .beta.2B, respectively. The sequence of IFN-.beta.2A cDNA is established. These genes and cDNA have been cloned into mammlian cells with an SV40 early promoter sequence and such genomic clones are capable of pro- ducing IFN-.beta.2A and IFN-.beta.2B. The antaviral activity of such recombinant IFN-.beta.2A and IFN-.beta.2B is demonstrated as well as other biological activity identifying them as human interferons. It has been shown that IFN-.beta.2 secretion is induced in human cells by growth- stimulatory cytokines.