Method for preparing a human monoclonal antibody, fragments thereof or antibodies comprising such fragments, resulting antibodies and use thereof

摘要

The invention concerns a method for preparing human monoclonal antibodies or fragments thereof or antibodies comprising such a fragment, from B lymphocytes. The invention is characterised in that it comprises the following steps: a) isolating B lymphocytes from a sample derived from a human subject; b) differentiating said B lymphocytes in plasma cells with at least a non-specific activating system and at least a cytokine; c) immortalising said plasma cells by cell fusion with a fusion line; d) culturing in vitro the fused cells until hybridomas are obtained; e) purifying the monoclonal antibodies produced by the hybridomas; f) optionally cleaving said antibodies into fragments and purifying one or several said fragments; g) optionally combining one or several fragments obtained at step f) with fragments of other antibodies.