METHOD FOR DETECTING METHYLATED CPG-CONTAINING NUCLEIC ACID

摘要

This invention concerns a method for detecting methylated CpG-containing nucleic acid by, contacting a nucleic acid containing specimen with an agent that modifies unmethylated cytosine; amplifying the nucleic acid region that contains the potential methylated CpG nucleic acid; separating and then reannealing the strands of the amplified DNA products; and detecting the presence of methylated nucleic acid based on duplex formation analysis. The method is particularly useful for prognosing, diagnosing or monitoring diseases associated with the methylation of nucleic acid, for example, tumours and certain developmental disorders.