PROCESS FOR THE PREPARATION OF PHOSPHOROTHIONATE OLIGONUCLEOTIDES

摘要

A process for the synthesis of phosphorothioate oligonucleotides is provided which comprises assembling an aligonucleotide bound to to a solid support in the presence of acetonitrile; prior to cleaving the oligonucleotide from the solid support removing the acetonitrile; and cleaving the oligonucleotide from the solid support. The process is particularly suited to the large scale synthesis of nucleotides. The acetonitrile may be removed from the solid support by one or both to drying and by washing with solvents. Preferred washing solvents comprise trialkylamines.