Protein and DNA sequence for pinosylvine-3-O-methyltransferase (PMT)

摘要

A recombinant plant protein (I), comprising the fully defined 400 amino acid sequence, its homologs or fragments, and having pinosylvin-3-O-methyltransferase (PMT) activity, is new. Independent claims are also included for the following: (1) a purified and isolated pinosylvin-3-O-methyltransferase gene (II) and its functional fragment, encoding (I); (2) an antisense sequence (III) to (II); (3) a recombinant expression vector (IV) containing (II) and/or (III); (4) a cDNA sequence (V) of (I); (5) an RNA sequence (VI) derived from (II), (III) or (V); (6) a prokaryotic or eukaryotic cell (VII) containing (II), (III) and/or (V); (7) a transgenic plant (VIII) containing (II), (III) and/or (V), including plant parts and propagation material such as protoplasts, cells, seeds, tubers and cuttings and progeny; and (8) preparing (VIII) and transgenic plant cells to express recombinant (I).