| 摘要 |
A E. coli recombinant plasmid expressing a fusion protein having the human erythropoietin receptor extracellular domain is disclosed. A puri- fied fusion protein produced from such a vector is also disclosed, the fusion protein having a cleav- age site suitable for separating the erythropoietin receptor extracellular domain from the remainder of the fusion protein. Antibodies having specific binding affinity for a purified extracellular domain polypeptide are also disclosed. The purified hu- man erythropoietin receptor fragment polypeptide binds erythropoietin. The articles, compositions and methods of the invention are useful for study- ing ligand binding to erythropoietin receptor and for quantitating the amounts of erythropoietin re- ceptor, as well as for understanding receptor struc- ture and signal transduction.
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