| 摘要 |
The invention relates to methods for the evolution of molecules with improved biological properties. In particular, the invention relates to methods using proteins that modify a DNA substrate to establish a link between the action of these proteins and the selection of molecules with improved biological properties. A unifying feature of all these mechanisms is that the coding region being evolved is in the same genetic element or on the same DNA molecule as a target site for a DNA-modifying protein. Accordingly, the activity (or inactivity) of the DNA-modifying protein can be tested by evaluating the sequence of its nucleic acid substrate. In this manner, a number of different types of compounds may be selected, including improved DNA-modifying proteins, improved substrates for DNA-modifying proteins, improved ligand-receptor interactions, improved co-factor and regulatory protein activities, improved DNA-binding proteins, and so on. |
