发明名称 |
Method for detecting a nucleotide sequence |
摘要 |
A method is disclosed for detecting a nucleotide sequence by a polymerase chain reaction, the nucleotide sequence is a constituent of a double-stranded nucleic acid molecule which is in solution and formed of a strand and a counter-strand. A first primer is added to the solution and is complementary to a first segment of the strand located at the 5'-terminus of a central segment. A second primer is added and is complementary to a 5'-terminal second segment of the counter-strand. The solution is brought into contact with a third primer whose 5'-terminal end is bound to a solid phase. The third primer is complementary to the central segment of the strand containing the nucleotide sequence. A reaction vessel is closed which contains the solution. The solution is repeatedly heated and cooled and the nucleic acid molecule is detected in the closed reaction vessel.
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申请公布号 |
US6403339(B1) |
申请公布日期 |
2002.06.11 |
申请号 |
US20000646575 |
申请日期 |
2000.11.16 |
申请人 |
NOVEMBER AKTIENGESELLSCHAFT GESELLSCHAFT FUER MOLEKULARE MEDIZIN |
发明人 |
BERTLING WOLF |
分类号 |
C12Q1/68;(IPC1-7):C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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