发明名称 |
Eukaryotic expression libraries and methods of use |
摘要 |
The invention provides a cell composition comprising a population of non-yeast eukaryotic cells containing a diverse population of variant nucleic acids, each of the variant nucleic acids being expressed in a different cell and located within each cell at an identical site in the genome. The invention also provides a method of identifying a polypeptide exhibiting optimized activity by screening a population of non-yeast eukaryotic cells containing a diverse population of variant nucleic acids for an activity associated with a parent polypeptide of a diverse population of variant polypeptides encoded by the variant nucleic acids; and identifying a variant polypeptide exhibiting an optimized activity relative to the parent polypeptide. |
申请公布号 |
AU3936902(A) |
申请公布日期 |
2002.06.11 |
申请号 |
AU20020039369 |
申请日期 |
2001.11.28 |
申请人 |
APPLIED MOLECULAR EVOLUTION, INC. |
发明人 |
WILLIAM D. HUSE |
分类号 |
G01N33/50;C12N1/15;C12N5/02;C12N5/10;C12N15/09;C12N15/10;C12Q1/02;C12Q1/68;G01N33/15;G01N33/53;G01N33/566;G01N33/68 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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