STEEP SOLVENT GRADIENT NMR ANALYSIS METHOD

摘要

An NMR method of analyzing am analyte comprises feeding an analyte sample fluid to an NMR flow cell. The NMR flow cell comprises an RF microcoil operably associated with an enlarged containment region. The mobile phase of the analyte sample flowing through the NMR flow cell has a solvent gradient than 10 % per minute. The analyte sample fluid can be fed to the NMR flow cell from an analyte extraction chamber, e.g., operative to perform liquid chromatography, capillary electrophoresis, or the like, especially a capillary-based analyte extraction chamber integrated in an NMR probe with the NMR flow cell. A sample volume is held in the NMR flow cell for equilibration less than 1 hour, preferably less than 30 minutes prior to actuating NMR analysis of the observe volume in the microcoil.