发明名称 |
STABLE SYSTEM FOR QUANTITATIVE DETERMINATION OF NF- B EXPRESSION IN HUMAN SKIN CELL AND USE THEREOF |
摘要 |
PURPOSE: Provided are stable system for quantitative determination of NF-kappa B expression in human skin cells and its use in screening skin whitening agents, anti-inflammatory agents or anti-oxidants. The analytical system uses fluorescent substrate to make it very sensitive and simple and is used effectively in screening skin whitening agents, anti-inflammatory agents or anti-oxidants in natural products and organic compounds. CONSTITUTION: The determination system comprises: a fused expression vector, pNF-k B-SEAP-NPT from fusing a gene expressing NF-kappa B transcription reporter, selected from SEAP(secretory alkaline phosphatase), luciferase, beta-galactosidase and GFP(green fluorescent protein), with a selectable marker expression gene chosen from NPT(neomycin phosphotransferase), CAT(chloramphenicol acetyltransferase) and beta-galactosidase, lacZ; transfecting it to skin cell line; and quantitatively determining the expression of NF-kappa B from the above transfected skin cell line(KCLRF-BP-00035) by fluorometry.
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申请公布号 |
KR20020040494(A) |
申请公布日期 |
2002.05.30 |
申请号 |
KR20000070557 |
申请日期 |
2000.11.24 |
申请人 |
KIM, YOUNG SIK;LEE, JIN SEON |
发明人 |
AHN, GWANG SEOK;HAN, BEOM SU;KIM, YOUNG SIK;LEE, JIN SEON;MUN, GI YEONG |
分类号 |
C12N15/65;(IPC1-7):C12N15/65 |
主分类号 |
C12N15/65 |
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