| 摘要 |
This invention is directed to the use of focused acoustic energy in the spatially directed ejection of cells suspended in a carrier fluid, for printing and patterning cells onto a substrate surface, for example to pattern an array of cells onto a substrate. An array of cells on a substrate surface comprising an array of substantially planar sites, with each site containing a single cell, is consequently also provided. Also disclosed are methods for the systematic generation and screening of arrays of living cells on a substrate from fluids containing one or more living cells. A method of attaching cells displaying a specific marker moiety on their surface, through specific recognition of the marker moiety by a cognate moiety that is linked to the surface is provided. Cells may be transformed to display a specific marker recognized by a corresponding cognate moiety, or the marker moiety may appear on untransformed cells. Cells displaying marker moieties may be conveniently attached to a surface functionalized with the cognate moiety. The combination of acoustic ejection and the marker moiety/cognate moiety system can be employed to select cells displaying a specific marker for adhesion to a substrate surface. The combination of several different marker moieties uniquely displayed on the cell surface of different types of cells combined with an array of different cognate moieties on a substrate may be employed in conjunction with the acoustic ejection of single living cells to create cell arrays with a specific number of cells displaying a specific marker attached to the substrate surface at a desired locale or region.
|
