发明名称 |
POSITION DEPENDENT RECOGNITION OF GNN NUCLEOTIDE TRIPLETS BYZINC FINGERS |
摘要 |
The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5'-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
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申请公布号 |
CA2429555(A1) |
申请公布日期 |
2002.05.30 |
申请号 |
CA20012429555 |
申请日期 |
2001.11.20 |
申请人 |
SANGAMO BIOSCIENCES, INC. |
发明人 |
LIU, QIANG;REBAR, EDWARD;JAMIESON, ANDREW |
分类号 |
C07K14/47;C12N15/10;C12N15/12;(IPC1-7):C12N15/12 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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