| 摘要 |
Isolation, enhanced yield, and maintenance ( e.g. ), culture) methods for cells, e.g. , cardiomyocytes, which maintain the structural and functional characteristics of freshly isolated cells are disclosed. Such methods can be used for developing longterm maintenance (cultures of cardiac myocytes which can be used in, for example, in vitro gene transfer, protein expression studies, and small molecule or drug screening, testing toxicology study. Optimized media solutions to enhance long-term survival of acutely isolated cells are also provided. |
