VECTOR AND METHOD FOR TARGETED REPLACEMENT AND DISRUPTION OF AN INTEGRATED DNA SEQUENCE

摘要

Cloning system in which the gene of interest is isolated and placed under the control of an inducible promoter. The system includes the ability to select for recombinants using both drug-selectable martkers and sorting cells by virtue of a cocistronic optically-active marker linked to the query gene. The vector also contains recombinase target sites flanking the bicistronic cassette for inserting, deleting or substituting said cassette or another cassette.