STEP-WISE PROCESS TO RECOVER OR ELIMINATE BIOLOGICAL SUBSTANCES BY FLOTATION FROM UNDERLAYERED COLLOIDAL MEDIUM

摘要

The present invention relates to a method and kit for discontinuous density gradient centrifugation to separate a minimum of two substances in a sample. In the method and kit use is made of colloidal density media and low density solutions, such as buffer solutions. The sample is mixed with colloidal media and placed at the bottom of a centrifuge tube and desired substances flotate to the lower density media following centrifugation.