Method of and apparatus for obtaining fluorescence image

摘要

Exciting light is projected onto a specimen such as an organic body, and an intensity in at least one wavelength range of fluorescence emitted from the specimen upon excitation by the exciting light is detected. Image data representing a fluorescence image of the specimen is obtained through an operation based on the intensity of the fluorescence. Whether each of pixels forming the fluorescence image is adequate for said operation is determined on the basis of the intensity of the fluorescence of each pixel. The operation is carried out on adequate pixels, which have been determined to be adequate for the operation, to obtain values for the adequate pixels while values which will not visually adversely affect the part of the fluorescence image corresponding to the adequate pixels are allotted to inadequate pixels, which have been determined not to be adequate for said operation.