发明名称 |
Expression vector for fusion gene and method for producing immobilized enzyme |
摘要 |
The present invention enables immobilization of a useful protein, for example, a glycosyltransferase, onto the surface of a yeast cell without deteriorating its enzyme activity. The present invention provides a fusion gene expression vector which is characterized by containing a fusion gene that comprises a gene encoding a useful protein bound downstream of a gene encoding a yeast cell wall protein; a transformant yeast, which is transformed with the fusion gene expression vector; and an immobilized enzyme, which is characterized in that the expressed useful protein is immobilized on a yeast cell wall. |
申请公布号 |
EP1209235(A1) |
申请公布日期 |
2002.05.29 |
申请号 |
EP20010309792 |
申请日期 |
2001.11.21 |
申请人 |
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY |
发明人 |
ABE, HIROKO;SHIMMA, YOH-ICHI;JIGAMI, YOSHIFUMI |
分类号 |
C12N15/09;C07K14/395;C12N1/19;C12N9/10;C12N11/16;C12N15/54;C12P19/18;C12R1/645 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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