LIQUID CHROMATOGRAPHIC SEPARATION OF POLYNUCLEOTIDES

摘要

In one aspect, the invention provides a method for separating a mixture of polynucleotides, such as DNA or RNA, including: (a) applying the mixture to a polymeric separation medium having non-polar surfaces, wherein the surfaces are characterized by being substantially free from multivalent cations, such as metal ions, which are free to interfere with polynucleotide separation; and (b) eluting the mixture with a mobile phase containing organic solvent and counter ion agent. In the separation of single-stranded polynucleotides, improved separation is obtained at a temperature effective to fully denature secondary structure within the polynucleotides.