The invention relates to a method and system for evaluating an effect on the nervous system of a test drug by comparing the effect of such drug on AChE catalytic activity or isoform variance in the brain of a test animal following challenge by an AChE blocker (e.g. DFP) or a blocker of AChE and muscarinic receptors M1 and M2 (e.g. pyridostigmine) and comparing this effect with that of a known agent, preferably a non-selective muscarinic receptor blocker (e.g. scopolamine) or a specific M1 receptor blocker (e.g. pirenzepine).Also provided is a method of screening for a candidate drug that is a modulator of the expression of any one of AChE variants and isoforms by determining the effect of such drug on the translocation of an AChE isoform within a neuron.Further provided is a method of screening for a candidate drug aimed at affecting central nervous system properties which is a modulator of the interaction between AChE-R/RACK1/PKC.
申请公布号
WO0240994(A2)
申请公布日期
2002.05.23
申请号
WO2001IL01051
申请日期
2001.11.14
申请人
YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW;SOREQ, HERMONA;MESHORER, ERAN;SKLAN, ELLA;SHOHAM, SHAI