| 摘要 |
The invention provides a novel transferase that acts on a saccharide, as a substrate, composed of at least three sugar units wherein at least three glucose residues on the reducing end are linked alpha-1,4 so as to transfer the alpha-1,4 lingages to a alpha-1,alpha-1 linkages; a process for producing the transferase; a gene coding for the same; and a process for producing an oligosaccharide by using the same. Also provided are a novel amylase that has a principal activity of acting on a saccharide, as a substrate, composed of at least three sugar units wherein at least three sugar units on the reducing end side are glucose units and the linkage between the first and the second glucose units is alpha-1,alpha-1 while the linkage between the second and the third glucose units is alpha-1,4 so as to liberate alpha,alpha-trehalose by hydrolyzing the alpha-1,4 linkage and another activity of hydrolyzing the alpha-1,4 linkage within the molecular chain of the substrate and that liberates disaccharides and/or monosaccharides as the principal final products; a process for producing the amylase; a gene coding for the same; and a process for producing alpha,alpha-trehalose by using a combination of the transferase and the amylase.
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