PSYCHROPHILE MICROORGANISM FOR THE DEGRADATION OF DIESEL AND PROCESS FOR PREPARATION THEREOF

摘要

PURPOSE: A psychrophile microorganism for the degradation of diesel and process for preparation thereof are provided, therefore psychrophile microorganism can be effectively used in purifying the oil contaminated soil in winter. CONSTITUTION: The psychrophile microorganism Pseudomonas sp. CX3B(KCTC 18054P), which is capable of decomposing oil including diesel below 12 deg.C., is isolated from the soil. Its preparation process comprises the steps of: collecting samples from the various oil-contaminated soils; inserting the samples into a phosphate buffer solution, agitating and sonicating them at 4 deg.C for 1 minute; cultivating the sample solution in a medium containing diesel and crude oil as a sole carbon source at 5 deg.C and 12 deg.C; spreading the cultivated medium on an agar medium to select oil-decomposing colonies through the color change by 2,6-DCPIP(Dichlorophenol-Indophenol) reduction; and screening a microorganism showing the most excellent oil-decomposability and viability at low temperature.